During use, directly immerse the tissue into Lysis Buffer premixed with Proteinase Mix, incubate at 55°C for 15 minutes to lysis tissue, and then heat at 95°C for 5 minutes to inactivate Proteinase. After centrifugation, the supernatant of the lysate can be directly used as a template for PCR amplification.

The 2x Direct-PCR Mix (Dye plus) in this kit contains DNA Taq Polymerase, dNTPs and a highly optimized buffer system with superior amplification and anti-interference ability. In the PCR reaction, only the template and primers need to be added to carry out the amplification, which reduces operations such as pipe opening and pipetting, and significantly improves the detection throughput and reproducibility of results. The dye is also premixed in the mix, and loaded directly after the PCR reaction. The PCR product has a base A at the 3' end and can be directly cloned into a T vector. This kit has a wide range of applications, and is especially suitable for mouse genotyping, transgenic detection and gene knockout analysis.