The procedure of this fecal and soil RNA extraction kit successfully isolates RNA from tough-to-lyse bacteria, fungi, protozoa (protist), algae, etc. in soil, and host RNA from fecal samples. Samples are added to the ZR BashingBead Lysis Tube with an optimally designed S/F RNA Lysis Buffer where microbes are then lysed by bead beating to extract total RNA. The Zymo-Spin column technology included in this fecal and soil RNA extraction kit allows for quick filtration, genomic DNA removal from sample lysates, and isolation of the RNA. Zymo-Spin III-HRC Spin Filter separates RT-PCR inhibitors (e.g., humic acids, polyphenols, tannins) and the total RNA is concentrated using the Zymo-Spin IC Column with a minimum elution volume of ≥6 μl.
HIGHLIGHTS:
·High Quality: Extract intact high quality total RNA (including small/micro RNAs) from various soil, fecal, and water samples.
·Homogenization: Ultra-high density BashingBead technology for complete disruption of tough-to-lyse samples.
·Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.